The Coren lab has constructed an arrayed 115,000-member human genomic library in the P1 Artificial Chromosome (PAC) shuttle vector pJCPAC-Mam2 that can be propagated in both bacterial and human cells.  This library should serve as a valuable resource to validate potential disease genes identified by genome-wide association studies (GWAS) in human cell lines and in animal models.  We are interested in using members from this library to address biological questions.  One research focus is trying to eradicate various cancers by expressing the p53 tumor suppressor gene in human tumor cell lines.  My lab is also investigating  the optimal transfection reagent for potential gene therapy studies.